Coding
Part:BBa_K1085010:Design
Designed by: Claudio Tiecher Group: iGEM13_Groningen (2013-09-10)
RBS Start-codon EstA Strep-tag SpiderSilkSubunitE1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 650
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 262
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DNA sequence was codon-optimized for Bacillus subtilis.
Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.
Source
The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008) and are from the MaSp2 of the Argiope aurantia.
The DNA sequence was synthesized by Integrated DNA Technologies (IDT).
References
Brooks, A. E., Stricker, S. M., Joshi, S. B., Kamerzell, T. J., Middaugh, C. R., & Lewis, R. V. (2008). Properties of synthetic spider silk fibers based on Argiope aurantia MaSp2. Biomacromolecules, 9(6), 1506–10